SiCORE are 1 µm sized non-magnetic, monodisperse microspheres based on a silica matrix.
The advantage of silica microspheres compared to polystyrene microspheres are the inert and stable solid-phase silica matrix without autofluorescence and are autoclaved for sterile applications.
Furthermore through the higher mechanical and chemical stability, hydrophilic character, resistance to microbiological degradation and compatible with organic solvents is it useful for biochromatiography applications.
SiCORE-active-microspheres are useful for covalent binding of bioaffine ligands, such as proteins or antibodies, as a tool for diagnostic application, e.g. Agglutination Test (LAT), Lateral flow assays or Particle Capture ELISA.
See: Coupling-Protocols
| Application: | Activated particles for custom-made covalent binding of biological substances |
| Formulation: | suspension in ddH2O, autoclaved |
| Concentration: | 50 mg/ml |
| Particle size: | 1.0 µm |
| Functional Group | Product Number | Quantity (ml) | Price EUR / USD |
Activation / Coupling | Product Information | Reference Number |
|---|---|---|---|---|---|---|
SiCORE-Amine
-O-(CH2)3-NH2
|
6401-1 6401-5 |
1 5 |
35 / 46 140 / 182 |
Carbodiimide method Mannich reaction see coupling protocol: C2/3 |
 |
- |
|
SiCORE-Carboxyl
-O-(CH2)3-COOH
|
6402-1 6402-5 |
1 5 |
35 / 46 140 / 182 |
Carbodiimide method see coupling protocol: C1 |
|
- |
|
SiCORE-Hydrazide
-O-(CH2)3~CO-NH-NH2
|
6404-1 6404-5 |
1 5 |
35 / 46 140 / 182 |
Oxidized carbohydrates see coupling protocol: C5 |
|
- |
|
SiCORE-Silanol
-Si-OH
|
6408-1 6408-5 |
1 5 |
35 / 46 140 / 182 |
Binding of DNA/RNA, adsorption of biomolecules | |
- |
|
SiCORE-Hydroxyl
-Si-(CH2)3-OH
|
6405-1 6405-5 |
1 5 |
35 / 46 140 / 182 |
Cyanogen bromide activation see coupling protocol: A7 |
|
- |
SiCORE-PGL
-Si-(CH2)3
|
6406-1 6406-5 |
1 5 |
35 / 46 140 / 182 |
For covalent coupling of bioligands see protocol: A4 |
|
- |
|
SiCORE-Thiol
-Si-(CH2)3-SH
|
6407-1 6407-5 |
1 5 |
35 / 46 140 / 182 |
Maleimides see coupling protocol: A6 |
|
- |
SiCORE-affinity - bioaffine ligands are covalently bound to the microspheres. High selectivity and high capacity make these products for an ideal tool for the binding of specific biomolecules.
See: Coupling-Protocols
| Application: | Affinity chromatography |
| Formulation: | buffer suspension, 0.01 NaN3 |
| Concentration: | 10 mg/ml |
| Particle sizes: | 1.0 µm |
| Affinity Group | Product Number | Quantity (ml) | Price EUR / USD |
For Purification | Product Information | Reference Number |
|---|---|---|---|---|---|---|
| SiCORE-Biotin | 6503-1 6503-5 |
1 5 |
55 / 72 220 / 286 |
Avidin / streptavidin labeled molecules | |
- |
| SiCORE-Boronic acid | 6504-1 6504-5 |
1 5 |
55 / 72 220 / 286 |
Ribonucleotides, sugars via cis-diols see protocol: CB14 |
|
- |
| SiCORE-Concanavalin A | 6506-1 6506-5 |
1 5 |
55 / 72 220 / 286 |
Bacteria, glycoproteins | |
- |
| SiCORE-Goat anti-rabbit IgG (whole molecule) |
6520-1 6520-5 |
1 5 |
55 / 72 220 / 286 |
For binding of secondary antibody See protocol: CB11 |
|
- |
| SiCORE-Goat anti-mouse IgG (whole molecule) |
6510-1 6510-5 |
1 5 |
55 / 72 220 / 286 |
For binding of secondary antibody See protocol: CB4 |
|
- |
| SiCORE-Protein A | 6515-1 6515-5 |
1 5 |
55 / 72 220 / 286 |
Antibodies See protocol: CB2 |
|
- |
| SiCORE-Protein G | 6516-1 6516-5 |
1 5 |
55 / 72 220 / 286 |
|
- | |
| SiCORE-Streptavidin | 6517-1 6517-5 |
1 5 |
55 / 72 220 / 286 |
Biotin-labeled molecules See protocol: CB1 |
|
- |
