Covalent Coupling Procedure on beadBALL-Hydrazide

This procedure describes the covalent coupling of aldehyde or ketone group containing ligands by the formation of stable hydrazone linkages on beadBALL-Hydrazide.

Glycoproteins can be immobilized by oxidation with sodium periodate to generate formyl groups on their carbohydrate chains.

This coupling method is a powerful way to immobilize proteins and leave critical active sites free.