SiMAG - Streptavidin

This procedure describes the binding of biotinylated ligands such as proteins, peptides or DNA on the SiMAG-Streptavidin beads.

The high association constant of Streptavidin / Biotin (10¹⁵ M^-1) guarantee a stable binding of biotinylated compounds with the correct orientation.

SiMAG-Streptavidin are a useful tool for molecular biological application such as isolation of specific DNA sequences and hybrids, cDNA subtraction, or capture of cycle sequencing or PCR reaction products and the isolation of mRNA. Furthermore is applicable for immuno- or protein-based methods or isolation of whole cells, enzyme complexes or organelles.