Protocol for isolation of vicinal cis-diol containing sugar compounds with beadBALL-Boronic acid

beadBALL-Boronic acid microspheres represent an easy-to-use affinity support for binding of glycoproteins, sugars, ribonucleotides and other small molecular weight compounds which contain vicinal cis-diol groups.

The immobilized boronic acid bind to vicinal cis-diol groups on sugars, such as mannose, galactose, glucose or ribose. After the binding and washing step the sugar containing sample can be eluted from the particles by lowering the pH or by addition of sorbitol.