SiMAG - Carboxyl

This procedure describes covalent coupling of amino-group containing ligands such as antibodies, proteins or low molecular substances to SiMAG-Carboxyl by the carbodiimide method.

The carbodiimide method is a binary covalent binding system and guarantees therefore a good reproducibility of the immobilization.

Carbodiimides react with the terminal carboxylate-groups from the magnetic beads to highly reactive O-acylisourea derivatives and react readily with amino-groups of the ligands.