Protocol for purification of IgG molecules with beadBALL-Protein A or beadBALL-Protein G

This protocol describes a quick enrichment procedure of IgG molecules from serum, ascites or cell culture supernatants by means of beadBALL-Protein A or beadBALL-Protein G.

Protein A / G is covalently coupled to the microspheres to provide an efficient tool for purifying different IgG antibodies.

beadBALL-Protein A / G binds with high specifity to the Fc region of most IgG subclasses from different species.