Covalent Coupling Procedure on beadBALL-Carboxyl via Carbodiimide Method

This procedure describes the covalent coupling of amino group containing ligands, such as antibodies, proteins or low molecular substances to beadBALL-Carboxyl by the carbodiimide method.

The carbodiimide method is a binary covalent binding procedure and guarantees a good reproducibility of the immobilization.

Carbodiimides react readily with the terminal carboxylate groups of the microspheres to highly reactive O-acylisourea derivatives, which then react readily with amino groups of the ligands.